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hmoB [2019-09-03 11:00:02]
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hmoB [2019-09-03 11:00:02]

heme monooxygenase
locus
BSU_10100
pI
5.22
mw
18.68 kDa
protein length
166 aa Sequence Blast
gene length
501 bp Sequence Blast
function
degradation of heme, acquisition of iron
product
heme monooxygenase
essential
no
synonyms
yixC,yhgC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]

    • Gene

    Coordinates
    1,083,229 1,083,729

    The protein

    Catalyzed reaction/ biological activity

  • binds hemin ''in vitro'' with ~1:1 stoichiometry and degrade hemin in the presence of an electron donor [Pubmed|21873409]
  • heme b + 3 O2 + 3 reduced [NADPH—hemoprotein reductase] --> biliverdin IXα + CO + Fe2+ + H+ + 3 H2O + 3 oxidized [NADPH—hemoprotein reductase] (according to UniProt)

    • Protein family

  • antibiotic biosynthesis monooxygenase family (with [protein|A4E1A1FA886980C863919DA011AC1EEDDBD74967|HmoA], according to UniProt)

    • Structure

  • [PDB|3TVZ] [Pubmed|22531134]

    • Expression and Regulation

    Operons

    genes
    [gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]
    description
    [Pubmed|8335642]

    regulation

  • constitutively expressed [Pubmed|21873409]
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    Biological materials

    Mutant

  • MGNA-B503 (yhgC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1502 NBRP B. subtilis, Japan]
  • BKE10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT, downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC
  • BKK10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT, downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC

    • References

  • 8335642,22531134,24582752,21873409